Composite

Part:BBa_K2404022

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-10-16)


TSH antagonist under control of the GST6 promoter

This construct contains the GST6 promotor, the TSHH coding sequence. We have not fully sequenced the 3' region of the construct but our PCR analysis indicates that it correctly includes the Nos Terminator.


This part is contained within the Phytobrick level 1 pGB-A2 plasmid.
The GST6 promoter is induced by the plant hormone salicylic acid..


Sequence and Features
1-6: BsmBI
16-513: PR2
514-1326: TSHH
1327-1593: NosT
1603-1608: BsmBI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 72
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1300
    Illegal XhoI site found at 1112
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 72
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 72
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None